Rail services to and from Westport, Co Mayo have been suspended after the second of separate collisions between trains and vehicles on the line today.
Irish Rail said that the 6.15pm Heuston to Westport service collided with a vehicle at a user-operated level crossing in Ballyhaunis, Co Mayo tonight.
The train operator said that emergency services were responding to attend to a number of occupants of the car.
Irish Rail added that its teams were working to arrange bus transfers for passengers, but that the line would remain until further notice to facilitate the emergency services response, and it warned that services in the morning may be affected.
Earlier this afternoon, the 1.10pm Westport to Dublin Heuston train struck a tractor that was crossing the line in Co Offaly, around 3.50pm.
Gardaí said emergency services attended the scene of the incident at the Meelaghans near Tullamore, and that a man had been taken to hospital for treatment of non-life-threatening injuries.
Bus transfers were in operation and the Galway line was expected to reopen tonight.